Mouse IL-13Ra2 Protein (ILR-MM1R2)

Immobilized Mouse IL-13Ra2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).

Mouse IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Mouse IL-13R2, His Tag with an affinity constant of 1.88 nM as determined in BLI assay .

The purity of Mouse IL-13Ra2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse IL-13Ra2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Amino Acid Range: Leu22-Lys334

Catalog #ILR-MM1R2

Price

$330⁰⁰

Size

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Mouse IL-13Ra2 Protein (ILR-MM1R2) Summary

Product Description

Recombinant Mouse IL-13Ra2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Leu22-Lys334.

Product Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Mouse IL-13Ra2 Protein (ILR-MM1R2) Specifications/Details

Protein	IL-13Ra2
Synonyms	CD213a2; IL-13 R alpha 2; IL13BP; IL13R; IL13RA2; IL-13Ra2;IL13R alpha 2
Accession	O88786-1
Species	Mouse
Biotinylated	no
Amino Acid Range	Leu22-Lys334
Molecular Weight	The protein has a predicted MW of 37.55 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse IL-13Ra2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Mouse IL-13Ra2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA (QC Test).
	Mouse IL-13Ra2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-13, hFc Tag on ProA-Biosensor can bind Mouse IL-13R2, His Tag with an affinity constant of 1.88 nM as determined in BLI assay .
	The purity of Mouse IL-13Ra2 is greater than 95% as determined by SEC-HPLC.