Mouse IL-17A&F Protein, Ultra Low Endotoxin (IAF-MM117-UL)

Express System: HEK293
Product Tag: N-Strep-II, N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Amino Acid Range: Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)

Catalog #IAF-MM117-UL

Price

$363.00

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Mouse IL-17A&F Protein, Ultra Low Endotoxin (IAF-MM117-UL) Summary

Product Description	Recombinant Mouse IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a Strep II tag at the N-terminus and subunit IL-17F is fused with a His tag at the N-terminus.It contains Ala26-Ala158 (IL-17A) and Arg29-Ala161 (IL-17F).
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Protein	IL-17A&F
Synonyms	IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
Accession	Q62386(IL-17A)&Q7TNI7-1(IL-17F)
Species	Mouse
Biotinylated	no
Amino Acid Range	Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)
Molecular Weight	The protein has a predicted MW of 16.01 kDa (IL-17A) and 15.98 kDa (IL-17F). Due to glycosylation, the protein migrates to 13-23 kDa and 25-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Product Tag	N-Strep-II, N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
	Mouse IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse IL-17A&F is greater than 95% as determined by SEC-HPLC.

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