Mouse IL-17A&F Protein (IL7-MM4AF)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)

Catalog: IL7-MM4AF

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Product Description Recombinant Mouse IL-17A&F Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala26-Ala158(IL-17A)&Arg29-Ala161(IL-17F).
Product Category Recombinant Protein
Target IL-17A&F
Synonyms IL-17A;Interleukin-17A;CTLA-8;IL-17;IL-17F;IL24;ML-1;Interleukin-17F
Gene/Protein ID Q62386(IL-17A)&Q7TNI7-1(IL-17F)
Species Mouse
Exact Sequence Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F)
Molecular Weight The protein has a predicted MW of 17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA.
The purity of Mouse IL-17A&F is greater than 95% as determined by SEC-HPLC.
Mouse IL-17A&F on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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