Mouse IL-17F Protein (ILF-MM417)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse IL-17F, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Arg29-Ala161

Catalog: ILF-MM417

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Product Description	Recombinant Mouse IL-17F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg29-Ala161.
Product Background	The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
Product Category	Recombinant Protein

Target	IL-17F
Synonyms	Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
Gene/Protein ID	Q7TNI7-1
Species	Mouse
Exact Sequence	Arg29-Ala161
Molecular Weight	The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse IL-17F, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse IL-17F, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA.
	The purity of Mouse IL-17F is greater than 95% as determined by SEC-HPLC.
	Mouse IL-17F on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.