Mouse IL-18BP Protein (IL8-MM1BP)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse IL-18BP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 23.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr29-Ala193

Catalog: IL8-MM1BP

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Product Description Recombinant Mouse IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr29-Ala193.

Product Background

Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.

Product Category

Recombinant Protein

Target

IL-18BP
Synonyms IL-18BP; Igifbp
Accession

Species

Mouse

Amino Acid Range

Thr29-Ala193
Molecular Weight The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse IL-18BP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 23.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-18BP, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.86 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse IL-18BP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 23.8ng/ml determined by ELISA (QC Test).
Mouse IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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