Mouse IL-18BP Protein (IL8-MM2BP) Summary
Product Description |
Recombinant Mouse IL-18BP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Thr29-Ala193. |
Product Background |
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse IL-18BP Protein (IL8-MM2BP) Specifications/Details
Protein |
IL-18BP |
Synonyms |
IL-18BP; Igifbp |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Thr29-Ala193 |
Molecular Weight |
The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-18BP, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 8.2ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Mouse IL-18BP, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 8.2ng/ml determined by ELISA. |
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Mouse IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IL-18BP is greater than 95% as determined by SEC-HPLC. |
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