- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Phe14-Glu355
Product Description |
Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe14-Glu355. |
Product Background |
Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-1R2/IL-1 RII/CD121b |
Synonyms |
IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Phe14-Glu355 |
Molecular Weight |
The protein has a predicted MW of 39.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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