Mouse IL-2 Protein (IL2-MM401)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala21-Gln169
Catalog #IL2-MM401
Regular price $300.00
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Product Description

Recombinant Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala21-Gln169.

Product Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-2

Synonyms

IL2; T-cell growth factor; TCGF; Aldesleukin

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ala21-Gln169

Molecular Weight

The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 1-5 ng/mL. Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 1-5 ng/mL.
The purity of Mouse IL-2 is greater than 95% as determined by SEC-HPLC. The purity of Mouse IL-2 is greater than 95% as determined by SEC-HPLC.

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