Mouse IL-2 Protein (IL2-MM401)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala21-Gln169

Catalog: IL2-MM401

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Product Description Recombinant Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala21-Gln169.

Product Background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

Product Category

Recombinant Protein

Target

IL-2
Synonyms IL2; T-cell growth factor; TCGF; Aldesleukin
Accession

Species

Mouse

Amino Acid Range

Ala21-Gln169
Molecular Weight The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 1-5 ng/mL.
The purity of Mouse IL-2 is greater than 95% as determined by SEC-HPLC.
Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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