Mouse IL-2 R alpha/CD25 Protein (CD5-MM125)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Glu22-Lys236
Catalog #CD5-MM125
Regular price $300.00
/
Size

Order now, ships by:

Product Description
Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
Product Background
IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category
Recombinant Protein
Target
IL-2 R alpha/CD25
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Glu22-Lys236
Molecular Weight
The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
Mouse IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed