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Mouse IL-2 R alpha/CD25 Protein (CD5-MM125) Summary
Product Description |
Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236. |
Product Background |
IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Mouse IL-2 R alpha/CD25 Protein (CD5-MM125) Specifications/Details
Protein |
IL-2 R alpha/CD25 |
Synonyms |
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu22-Lys236 |
Molecular Weight |
The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). |
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Mouse IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IL-2 R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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