Mouse IL-2 R alpha/CD25 Protein (CD5-MM125)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu22-Lys236

Catalog: CD5-MM125

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Product Description	Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236.
Product Background	IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category	Recombinant Protein

Target	IL-2 R alpha/CD25
Accession	P01590
Species	Mouse
Amino Acid Range	Glu22-Lys236
Molecular Weight	The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
	The purity of Mouse IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
	Mouse IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.