- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Glu22-Lys236
Product Description |
Recombinant Mouse IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Lys236. |
Product Background |
IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Product Category |
Recombinant Protein |
Target |
IL-2 R alpha/CD25 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Glu22-Lys236 |
Molecular Weight |
The protein has a predicted MW of 25.76 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-2 R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 30.49 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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