- Express System: E.coli
- Product Tag: N-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Leu34-Val179
Product Description |
Recombinant Mouse IL-22 Protein is expressed from E.coli with His tag at the N-terminus.It contains Leu34-Val179. |
Product Background |
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production. |
Product Category |
Recombinant Protein |
Target |
IL-22 |
Synonyms |
Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu34-Val179 |
Molecular Weight |
The protein has a predicted MW of 17.73 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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