Mouse IL-22 Protein (IL2-ME122)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu34-Val179
Catalog #IL2-ME122
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Product Description
 Recombinant Mouse IL-22 Protein is expressed from E.coli with His tag at the N-terminus.It contains Leu34-Val179.
Product Background
 The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Product Category
 Recombinant Protein
Target
 IL-22
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Leu34-Val179
Molecular Weight
 The protein has a predicted MW of 17.73 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
Mouse IL-22 Protein on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-22 is greater than 95% as determined by SEC-HPLC.

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