Product Description |
Recombinant Mouse IL-22 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Leu34-Val179. |
Product Background |
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production. |
Product Category |
Recombinant Protein |
Target |
IL-22 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu34-Val179 |
Molecular Weight |
The protein has a predicted MW of 17.73 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test). |
|
|
Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse IL-22 is greater than 95% as determined by SEC-HPLC. |
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