Mouse IL-23 alpha&IL-12 beta Protein (IL2-MM1AB)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)
Catalog #IL2-MM1AB
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Product Description
 Recombinant Mouse IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val22-Ala196(IL-23 alpha)&Met23-Ser335(IL-12 beta).
Product Background
 Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Product Category
 Recombinant Protein
Target
 IL-23 alpha&IL-12 beta
Synonyms
 IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)
Molecular Weight
 The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
Mouse IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.

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