Mouse IL-23 alpha&IL-12 beta Protein (IL2-MM1AB)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)

Catalog: IL2-MM1AB

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Product Description Recombinant Mouse IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val22-Ala196(IL-23 alpha)&Met23-Ser335(IL-12 beta).
Product Category Recombinant Protein
Target IL-23 alpha&IL-12 beta
Synonyms IL23 alpha; IL12 beta; IL23 alpha&IL12 beta
Gene/Protein ID Q9EQ14-1(IL-23 alpha)&P43432-1(IL-12 beta)
Species Mouse
Exact Sequence Val22-Ala196(IL-23 alpha) & Met23-Ser335(IL-12 beta)
Molecular Weight The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 6.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).
The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Mouse IL-23 alpha&IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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