• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 85% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Tyr19-Pro228(IL-27B) & Phe29-Ser234(IL-27A)
Catalog #IL2-MM127
Price
Regular price $37500
Regular price Sale price $37500
/
Size
Avantor VWR Fisher Scientific

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Mouse IL-27 Protein (IL2-MM127) Summary

Product Description

 Recombinant Mouse IL-27 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr19-Pro228 (IL-27B) and Phe29-Ser234 (IL-27A).

Product Background

 Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.

Mouse IL-27 Protein (IL2-MM127) Specifications/Details

Protein
 IL-27
Synonyms
 Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28;;IL27A;IL 27; Interleukin-30; Interleukin 30; Interleukin 27
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Tyr19-Pro228(IL-27B) & Phe29-Ser234(IL-27A)
Molecular Weight
 The protein has a predicted MW of 49.58 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 85% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
Mouse IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%. Mouse IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
The purity of Mouse IL-27 is greater than 85% as determined by SEC-HPLC. The purity of Mouse IL-27 is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...