Mouse IL-27 Protein (IL2-MM127)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 85% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
  • Activity: Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Tyr19-Pro228(IL-27B) & Phe29-Ser234(IL-27A)
Catalog #IL2-MM127
Regular price $375.00
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Size
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Mouse IL-27 Protein (IL2-MM127) Summary

Product Description

Recombinant Mouse IL-27 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Tyr19-Pro228 (IL-27B) and Phe29-Ser234 (IL-27A).

Product Background

Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Mouse IL-27 Protein (IL2-MM127) Specifications/Details

Protein
IL-27
Synonyms
Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28;
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Tyr19-Pro228(IL-27B) & Phe29-Ser234(IL-27A)
Molecular Weight
The protein has a predicted MW of 49.58 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 85% as determined by Bis-Tris PAGE;> 85% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human IL-27RA, hFc Tag (Cat. ILR-HM127) at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-27, His Tag with the EC50 of 2.7ng/ml determined by ELISA.
Mouse IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%. Mouse IL-27 on Bis-Tris PAGE under reduced condition. The purity is greater than 85%.
The purity of Mouse IL-27 is greater than 85% as determined by SEC-HPLC. The purity of Mouse IL-27 is greater than 85% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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