Product Description |
Recombinant Mouse IL-3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala27-Cys166. |
Product Background |
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. |
Product Category |
Recombinant Protein |
Target |
IL-3 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala27-Cys166 |
Molecular Weight |
The protein has a predicted MW of 16.77 kDa. Due to glycosylation, the protein migrates to 27-40 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50-for this effect is 0.1 - 0.5 ng/mL. |
|
|
The purity of Mouse IL-3 is greater than 95% as determined by SEC-HPLC. |
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