Mouse IL-3 Protein (IL3-MM201) Summary
Product Description |
Recombinant Mouse IL-3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Cys166. |
Product Background |
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse IL-3 Protein (IL3-MM201) Specifications/Details
Protein |
IL-3 |
Synonyms |
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala27-Cys166 |
Molecular Weight |
The protein has a predicted MW of 42.2 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Mouse IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse IL-3, hFc Tag on ProA-Biosensor can bind Mouse IL-3 R alpha, His Tag with an affinity constant of 0.79 µM as determined in BLI assay (Gator® Prime). |
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The purity of Mouse IL-3 is greater than 95% as determined by SEC-HPLC. |
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