Product Description |
Recombinant Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Met21-Gly133. |
Product Background |
IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA). |
Product Category |
Recombinant Protein |
Target |
IL-5 |
Synonyms |
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met21-Gly133 |
Molecular Weight |
The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 16.03 kDa and 20-32 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.495 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). |
|
|
Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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