• Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.495 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met21-Gly133
Catalog #IL5-MM401
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Mouse IL-5 Protein (IL5-MM401) Summary

Product Description

 Recombinant Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Met21-Gly133.

Product Background

 IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(RĪ±) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Non-biotinylated Mouse IL-5 Protein (IL5-MM401) Specifications/Details

Protein
 IL-5
Synonyms
 Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Met21-Gly133
Molecular Weight
 The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 16.03 kDa and 20-32 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.495 uM as determined in SPR assay (Biacore T200).
Product Tag
 N-His-Avi

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80Ā°C.ļ¼› Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200). Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test). Immobilized Mouse IL-5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA (QC Test).
Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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