Product Description |
Recombinant Mouse IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp18-His339. |
Product Background |
Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5. |
Product Category |
Recombinant Protein |
Target |
IL-5 R alpha |
Synonyms |
CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Asp18-His339 |
Molecular Weight |
The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.495 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200). |
|
|
Mouse IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse IL-5 R alpha is greater than 95% as determined by SEC-HPLC. |
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