Mouse IL-7 Protein (IL7-MM107)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-7R alpha, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
  • Amino Acid Range: Glu26-Ile154
Catalog #IL7-MM107
Regular price $345.00
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Size
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Mouse IL-7 Protein (IL7-MM107) Summary

Product Description

Recombinant Mouse IL-7 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu26-Ile154.

Product Background

Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Mouse IL-7 Protein (IL7-MM107) Specifications/Details

Protein
IL-7
Synonyms
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Glu26-Ile154
Molecular Weight
The protein has a predicted MW of 16.51 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse IL-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-7R alpha, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse IL-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-7R alpha, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. Immobilized Mouse IL-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse IL-7R alpha, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
Mouse IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IL-7 is greater than 95% as determined by SEC-HPLC. The purity of Mouse IL-7 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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