HPLC chromatogram showing a primary protein peak at 9.865 minutes with minor secondary peaks indicating product purity.
Mouse IL-9-Protein (IL9-MM101)
Mouse IL-9-Protein (IL9-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gln19-Pro144
Catalog #IL9-MM101
Regular price $346.50
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Mouse IL-9-Protein (IL9-MM101) Summary

Product Description

Recombinant Mouse IL-9-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Pro144.

Product Background

IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse IL-9-Protein (IL9-MM101) Specifications/Details

Protein
IL-9
Synonyms
Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40; HP40; P40
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Gln19-Pro144
Molecular Weight
The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse IL-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse IL-9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is < 3 ng/mL. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is < 3 ng/mL.
The purity of Mouse IL-9 is greater than 95% as determined by SEC-HPLC. The purity of Mouse IL-9 is greater than 95% as determined by SEC-HPLC.
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