Product Description |
Recombinant Mouse LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu438. |
Product Background |
Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport. |
Product Category |
Recombinant Protein |
Target |
LCAT |
Synonyms |
PAF acetylhydrolase; LCAT |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Phe25-Glu438 |
Molecular Weight |
The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 67-75 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse LCAT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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