Mouse LCAT Protein (LAT-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Phe25-Glu438
Catalog #LAT-MM101
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Product Description
 Recombinant Mouse LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu438.
Product Background
 Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.
Product Category
 Recombinant Protein
Target
 LCAT
Synonyms
 PAF acetylhydrolase; LCAT
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Phe25-Glu438
Molecular Weight
 The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 67-75 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse LCAT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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