Product Description |
Recombinant Mouse Leptin Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val22-Cys167. |
Product Background |
Leptin has key roles in the regulation of energy balance, body weight, metabolism, and endocrine function. Leptin levels are undetectable or very low in patients with lipodystrophy, hypothalamic amenorrhea, and congenital leptin deficiency (CLD) due to mutations in the leptin gene. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Leptin |
Synonyms |
Leptin; Obese protein; Obesity factor; LEP; OB; OBS; LEPD |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val22-Cys167 |
Molecular Weight |
The protein has a predicted MW of 42.76 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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