Mouse Leptin Protein (LEP-ME201)
Catalog: LEP-ME201
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| Product Description | Recombinant Mouse Leptin Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val22-Cys167. |
Product Background |
Leptin has key roles in the regulation of energy balance, body weight, metabolism, and endocrine function. Leptin levels are undetectable or very low in patients with lipodystrophy, hypothalamic amenorrhea, and congenital leptin deficiency (CLD) due to mutations in the leptin gene. |
Product Category |
Recombinant Protein |
Target |
Leptin |
| Synonyms | Leptin; Obese protein; Obesity factor; LEP; OB; OBS; LEPD |
Gene/Protein ID |
P41160 |
Species |
Mouse |
Exact Sequence |
Val22-Cys167 |
| Molecular Weight | The protein has a predicted MW of 42.76 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-hFc |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Mouse Leptin is greater than 95% as determined by SEC-HPLC. |
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Mouse Leptin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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