Mouse LIF Protein (LIF-MM201) Summary
Product Description |
Recombinant Mouse LIF Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Pro25-Phe203. |
Product Background |
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse LIF Protein (LIF-MM201) Specifications/Details
Protein |
LIF |
Synonyms |
LIF; HILDA; MLPLI; CDF; DIA |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Pro25-Phe203 |
Molecular Weight |
The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse LIF is greater than 95% as determined by SEC-HPLC. |
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