Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly24-Lys238
Catalog #LIL-MM1B4
Regular price $300.00
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Product Description

Recombinant Mouse LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Lys238.

Product Background

LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

LILRB4/CD85k/ILT3

Synonyms

HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Gly24-Lys238

Molecular Weight

The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).
Mouse LILRB4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Mouse LILRB4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC. The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC.

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