Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4)
Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Gly24-Lys238
Catalog #LIL-MM1B4
Regular price $330.00
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Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4) Summary

Product Description

Recombinant Mouse LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Lys238.

Product Background

LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4) Specifications/Details

Protein
LILRB4/CD85k/ILT3
Synonyms
HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Gly24-Lys238
Molecular Weight
The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse LILRB4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Mouse LILRB4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC. The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC.
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