Product Description |
Recombinant Mouse M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Ser511. |
Product Background |
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. |
Product Category |
Recombinant Protein |
Target |
M-CSF R/CSF1R/CD115 |
Synonyms |
CD115; CSF1R; CSF-1-R; CSFR; FMSFIM2; M-CSF R; MCSFR; M-CSFR; C-FMS; FIM2; FMS; HDLS |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala20-Ser511 |
Molecular Weight |
The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 76-82 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA (QC Test). The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse M-CSF R, His tag captured on CM5 Chip via Anti-His Antibody can bind Human M-CSF, No tag with an affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Mouse M-CSF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse M-CSF R, His Tag with the EC50 of 2.18ug/ml determined by ELISA (QC Test). |
|
|
Mouse M-CSF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
