Mouse MASP3 Protein (MSP-MM113)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse MASP3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg.
Amino Acid Range: Ile455-Arg733

Catalog #MSP-MM113

Price

$525.00

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Mouse MASP3 Protein (MSP-MM113) Summary

Product Description	Recombinant Mouse MASP3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ile455-Arg733.
Product Background	Mannose-binding lectin-associated serine protease-1 (also named MASP3), a multifunctional serine protease, plays an important role in innate immunity which is capable of activating the lectin pathway of the complement system and also triggering coagulation cascade system.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Protein	MASP3
Synonyms	MASP-3; MASP-1; MASP1; RaRF; Serine protease 5; CRARF; CRARF1; PRSS5;
Accession	P98064-2
Species	Mouse
Biotinylated	no
Amino Acid Range	Ile455-Arg733
Molecular Weight	The protein has a predicted MW of 32.33 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse MASP3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse MASP3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
	Mouse MASP3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse MASP3 is greater than 95% as determined by SEC-HPLC.

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