Mouse MMP-9 Protein (pro form) (MMP-MM109)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂. The specific activity is > 800 pmol/min/µg.
Amino Acid Range: Ala20-Pro730

Catalog #MMP-MM109

Price

$330⁰⁰

Size

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Mouse MMP-9 Protein (pro form) (MMP-MM109) Summary

Product Description

Recombinant Mouse MMP-9 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Ala20-Pro730.

Product Background

Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14.

Mouse MMP-9 Protein (pro form) (MMP-MM109) Specifications/Details

Protein	MMP-9
Synonyms	MMP-9; GELB; CLG4B; MANDP2; Gelatinase B;MMP9
Accession	P41245
Species	Mouse
Biotinylated	no
Amino Acid Range	Ala20-Pro730
Molecular Weight	The protein has a predicted MW of 79.64 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH₂. The specific activity is > 800 pmol/min/µg.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 10mM CaCl₂, 150mM NaCl, 0.05% Brij-35 (w/v) (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.