Mouse Nectin-2/CD112 Protein (NEC-MM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test). The affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln32-Gly351
Catalog #NEC-MM102
Regular price $240.00
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Product Description

Recombinant Mouse Nectin-2/CD112 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Gly351.

Product Background

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

Product Category

Recombinant Protein

Protein

Nectin-2/CD112

Synonyms

CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Gln32-Gly351

Molecular Weight

The protein has a predicted MW of 35.7 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test). The affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). Mouse PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 0.355 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test). Immobilized Mouse Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.62ug/ml determined by ELISA (QC Test).
Mouse Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Nectin-2 is greater than 95% as determined by SEC-HPLC. The purity of Mouse Nectin-2 is greater than 95% as determined by SEC-HPLC.

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