Mouse Nectin-2/CD112 Protein (NEC-MM102)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln32-Gly351

Catalog: NEC-MM102

$240.00
Size:

Order now get it on

Product Description Recombinant Mouse Nectin-2/CD112 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Gly351.

Product Background

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

Product Category

Recombinant Protein

Target

Nectin-2/CD112
Synonyms CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2

Gene/Protein ID

P32507

Species

Mouse

Exact Sequence

Gln32-Gly351
Molecular Weight The protein has a predicted MW of 35.7 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). The affinity constant of 0.355 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse PVRIG, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 0.355 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Mouse PVRIG, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
The purity of Mouse Nectin-2 is greater than 95% as determined by SEC-HPLC.
Mouse Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in