Mouse NKG2D/CD314 Protein (NKG-MM12D)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 0.39ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe90-Val232

Catalog: NKG-MM12D

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Product Description	Recombinant Mouse NKG2D/CD314 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Phe90-Val232.
Product Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Product Category	Recombinant Protein

Target	NKG2D/CD314
Synonyms	CD314; D12S2489E; KLR; NKG2-D; NKG2D
Gene/Protein ID	O54709-1
Species	Mouse
Exact Sequence	Phe90-Val232
Molecular Weight	The protein has a predicted MW of 17.5 kDa. Due to glycosylation, the protein migrates to 24-35kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 0.39ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 250mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 0.39ug/ml determined by ELISA.
	The purity of Mouse NKG2D is greater than 95% as determined by SEC-HPLC.
	Mouse NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.