Mouse OX40 Ligand/TNFSF4 Protein (OXL-MM240)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse OX-40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser51-Leu198

Catalog: OXL-MM240

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Product Description Recombinant Mouse OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ser51-Leu198.

Product Background

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.

Product Category

Recombinant Protein

Target

OX-40 Ligand/TNFSF4
Synonyms OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34
Accession

Species

Mouse

Amino Acid Range

Ser51-Leu198
Molecular Weight The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 52-56 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse OX-40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse OX-40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA.
The purity of Mouse OX40 Ligand is greater than 95% as determined by SEC-HPLC.
Mouse OX40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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