Mouse P4HB Protein (PHB-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Asp20-Leu509

Catalog: PHB-MM101

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Product Description Recombinant Mouse P4HB Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp20-Leu509.

Product Background

 Protein disulfide isomerase (PDI, also named P4HB) is a 57-kDa oxidoreductase that facilitates cysteine thiol reactions inside and outside the cell. It mediates reduction or oxidation of protein disulfide bonds, thiol/disulfide exchange reactions, and transfer of NO from one protein thiol to another. It also has chaperone properties. PDI is actively secreted by most, if not all, of the cell types involved in thrombosis, binds to integrins on the cell surface, and circulates as a soluble protein in blood.

Product Category

 Recombinant Protein

Target

 P4HB

Gene/Protein ID

 P09103

Species

 Mouse

Exact Sequence

 Asp20-Leu509
Molecular Weight The protein has a predicted MW of 56.18 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A650/cm/min/mg.
The purity of Mouse P4HB is greater than 95% as determined by SEC-HPLC.
Mouse P4HB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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