HPLC chromatogram showing a main protein peak at retention time 8.979 minutes with a minor peak at 7.924 minutes for purity analysis.
Mouse P4HB Protein (PHB-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A650/cm/min/mg.
  • Amino Acid Range: Asp20-Leu509
Catalog #PHB-MM101
Regular price $297.00
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Size
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Mouse P4HB Protein (PHB-MM101) Summary

Product Description

Recombinant Mouse P4HB Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp20-Leu509.

Product Background

Protein disulfide isomerase (PDI, also named P4HB) is a 57-kDa oxidoreductase that facilitates cysteine thiol reactions inside and outside the cell. It mediates reduction or oxidation of protein disulfide bonds, thiol/disulfide exchange reactions, and transfer of NO from one protein thiol to another. It also has chaperone properties. PDI is actively secreted by most, if not all, of the cell types involved in thrombosis, binds to integrins on the cell surface, and circulates as a soluble protein in blood.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse P4HB Protein (PHB-MM101) Specifications/Details

Protein
P4HB
Synonyms
PDI; ERp59; p55; Pdia1; Thbp
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Asp20-Leu509
Molecular Weight
The protein has a predicted MW of 56.18 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is > 10 A650/cm/min/mg.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse P4HB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse P4HB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse P4HB is greater than 95% as determined by SEC-HPLC. The purity of Mouse P4HB is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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