Mouse PCSK9 Protein (PCS-MM190)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln35-Gln694

Catalog: PCS-MM190

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Product Description	Recombinant Mouse PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gln694.
Product Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.
Product Category	Recombinant Protein

Target	PCSK9
Synonyms	PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1
Gene/Protein ID	Q80W65
Species	Mouse
Exact Sequence	Gln35-Gln694
Molecular Weight	Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCL, 200mM L-Aginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.61 nM as determined in SPR assay (Biacore T200).
	The purity of Mouse PCSK9 is greater than 95% as determined by SEC-HPLC.
	Mouse PCSK9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.