Product Description |
Recombinant Mouse PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gln694. |
Product Background |
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated. |
Product Category |
Recombinant Protein |
Target |
PCSK9 |
Synonyms |
PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln35-Gln694 |
Molecular Weight |
Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.61 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCL, 200mM L-Aginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human LDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.61 nM as determined in SPR assay (Biacore T200). |
|
|
Mouse PCSK9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse PCSK9 is greater than 95% as determined by SEC-HPLC. |
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