Mouse PD-1/PDCD1 Protein (PD1-MM201)
Catalog: PD1-MM201
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| Product Description | Recombinant Mouse PD-1/PDCD1 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Leu25-Gln167. |
| Product Category | Recombinant Protein |
| Target | PD-1/PDCD1 |
| Synonyms | PDCD1; PD1; CD279; SLEB2; PD-1 |
| Gene/Protein ID | NP_032824 |
| Species | Mouse |
| Exact Sequence | Leu25-Gln167 |
| Molecular Weight | The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.15_M as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per _g by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse PD-L1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.15_M as determined in SPR assay (Biacore T200). |
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The purity of Mouse PD-1 is greater than 95% as determined by SEC-HPLC. |
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Mouse PD-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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