Mouse PD-L1/B7-H1 Protein (PDL-MM110)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: The affinity constant of 0.15μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe19-Thr238

Catalog: PDL-MM110

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Product Description Recombinant Mouse PD-L1/B7-H1 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Phe19-Thr238.
Product Category Recombinant Protein
Target PD-L1/B7-H1
Synonyms CD274; PDL1; PD-L1; PD-L1B7 homolog 1; B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Gene/Protein ID NP_068693
Species Mouse
Exact Sequence Phe19-Thr238
Molecular Weight The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.15μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse PD-L1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.15μM as determined in SPR assay (Biacore T200).
The purity of Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.
Mouse PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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