Mouse PD-L1/B7-H1 Protein (PDL-MM210)
Catalog: PDL-MM210
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| Product Description | Recombinant Mouse PD-L1/B7-H1 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Phe19-Arg238. |
| Product Category | Recombinant Protein |
| Target | PD-L1/B7-H1 |
| Synonyms | CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
| Gene/Protein ID | NP_068693 |
| Species | Mouse |
| Exact Sequence | Phe19-Thr238 |
| Molecular Weight | The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 92.49 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human PD-1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-L1, hFc Tag with an affinity constant of 92.49 nM as determined in SPR assay (Biacore T200). |
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The purity of Mouse PD-L1 is greater than 95% as determined by SEC-HPLC. |
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Mouse PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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