Mouse PGF Protein (PGF-MM101)
Mouse PGF Protein (PGF-MM101)
Mouse PGF Protein (PGF-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val19-Pro158
Catalog #PGF-MM101
Regular price $270.00
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Product Description

Recombinant Mouse PGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro158.

Product Background

Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.

Product Category

Recombinant Protein / Antibody discovery

Protein

PGF

Synonyms

PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Val19-Pro158

Molecular Weight

The protein has a predicted MW of 16.99 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test). Immobilized Mouse PGF, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGF R1, hFc Tag with the EC50 of 54.6ng/ml determined by ELISA (QC Test).
Mouse PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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