Name: Mouse PGF Protein (PGF-MM201)
Brand: KactusBio
SKU: PGF-MM201-100UG
Price: 429.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PGF, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
Amino Acid Range: Val19-Pro158

Catalog #PGF-MM201

Size

100UG

500UG

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Mouse PGF Protein (PGF-MM201) Summary

Product Description	Recombinant Mouse PGF Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val19-Pro158.
Product Background	Placental growth factor (PGF) is another member of the VEGF family of cytokines with pro-angiogenic and pro-inflammatory effects. Retinal inhibition of PGF in combination with VEGF-A prevents vascular leakage and CNV possibly via modulating their own expression in mononuclear phagocytes. PGF-related, optimized strategies to target inflammation-mediated angiogenesis may help to increase efficacy and reduce non-responders in the treatment of wet AMD patients.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Mouse PGF Protein (PGF-MM201) Specifications/Details

Protein	PGF
Synonyms	PlGF; PGF; PGFL; SHGC-10760; D12S1900; PlGF-2
Accession	P49764
Species	Mouse
Biotinylated	no
Amino Acid Range	Val19-Pro158
Molecular Weight	The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PGF, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse PGF, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
	Mouse PGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse PGF is greater than 95% as determined by SEC-HPLC.

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