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Mouse PGK1 Protein (PGK-MB101) Summary
Product Description |
Recombinant Mouse PGK1 Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Val417. |
Product Background |
Phosphoglycerate kinase 1 (PGK1) is the first critical enzyme to produce ATP in the glycolytic pathway. PGK1 is not only a metabolic enzyme but also a protein kinase, which mediates the tumor growth, migration and invasion through phosphorylation some important substrates. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse PGK1 Protein (PGK-MB101) Specifications/Details
Protein |
PGK1 |
Synonyms |
Pgk-1;Pgk 1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met1-Val417 |
Molecular Weight |
The protein has a predicted MW of 46.07 kDa same as Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
Baculovirus-Insect Cells(Sf9) |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 500mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse PGK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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