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Mouse PLA2G7 Protein, Ultra Low Endotoxin (PLA-MM107-UL) Summary
Product Description |
Recombinant Mouse PLA2G7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Asn440. |
Product Background |
Endogenous PLA2G7 transcription levels were found to be significantly lower in vascular related cell lines than in the other cell lines. Luciferase reporter gene assays indicated that gene activity was significantly enhanced by PLA2G7 promoter fragment. LA2G7 transcription was found to be up-regulated with the treatment of DAC. The 17-β-estradiol was found to down-regulate PLA2G7 transcription in all the cell lines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse PLA2G7 Protein, Ultra Low Endotoxin (PLA-MM107-UL) Specifications/Details
Protein |
PLA2G7 |
Synonyms |
LDL-PLA2; PAFAD; PAFAH |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Phe22-Asn440 |
Molecular Weight |
The protein has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse PLA2G7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse PLA2G7 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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