Mouse PLD4 Protein (PLD-MM104) Summary
Product Description |
Recombinant Mouse PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln58-Trp503. |
Product Background |
Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse PLD4 Protein (PLD-MM104) Specifications/Details
Protein |
PLD4 |
Synonyms |
5'-3' exonuclease PLD4; Choline phosphatase 4; Phospholipase D4; PLD 4 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln58-Trp503 |
Molecular Weight |
The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 100mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM MES, 100mM NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse PLD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse PLD4 is greater than 95% as determined by SEC-HPLC. |
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