Name: Mouse R spondin 1/RSPO1 Protein (RS1-MM101)
Brand: KactusBio
SKU: RS1-MM101-100UG
Price: 375.0 USD
Availability: InStock

Mouse R spondin 1/RSPO1 Protein (RS1-MM101)

Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.

Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser21-Gln265

Catalog #RS1-MM101

Size

100UG

500UG

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Product Description	Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Gln265.
Product Background	The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Product Category	Recombinant Protein / Antibody discovery

Protein	R spondin 1/RSPO1
Synonyms	R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1
Accession	Q9Z132
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser21-Gln265
Molecular Weight	The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.
	Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).