Product Description |
Recombinant Mouse R spondin 1/RSPO1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Gln265. |
Product Background |
The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
Product Category |
Recombinant Protein |
Protein |
R spondin 1/RSPO1 |
Synonyms |
R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ser21-Gln265 |
Molecular Weight |
The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5). |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Mouse R spondin 1, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. |
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Mouse R spondin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test). |
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