Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr70-Asp316

Catalog: RKL-MM101

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Product Description Recombinant Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr70-Asp316.

Product Background

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Product Category

Recombinant Protein

Target

RANKL/TNFSF11/CD254

Gene/Protein ID

AAC40113.1

Species

Mouse

Exact Sequence

Tyr70-Asp316
Molecular Weight The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC.
Mouse RANKL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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