Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
Mouse RANKL/TNFSF11/CD254 Protein (RKL-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr70-Asp316
Catalog #RKL-MM101
Regular price $315.00
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Product Description

Recombinant Mouse RANKL/TNFSF11/CD254 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr70-Asp316.

Product Background

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.

Product Category

Recombinant Protein / Antibody discovery

Protein

RANKL/TNFSF11/CD254

Synonyms

ODF; OPGL; RANKL; TRANCE; CD254; sOdf; TNFSF11; OPTB2

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Tyr70-Asp316

Molecular Weight

The protein has a predicted MW of 28.97 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
Mouse RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse RANKL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC. The purity of Mouse RANKL is greater than 95% as determined by SEC-HPLC.

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