Mouse RNF43 Protein (RNF-MM234)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly24-Tyr197

Catalog: RNF-MM234

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Product Description Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197.

Product Background

RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.

Product Category

Recombinant Protein

Target

RNF34
Synonyms E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;Rnf43
Accession

Species

Mouse

Exact Sequence

Gly24-Tyr197
Molecular Weight The protein has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA.
The purity of Mouse RNF43 is greater than 95% as determined by SEC-HPLC.
Mouse RNF43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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