Product Description |
Recombinant Mouse RNF43 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly24-Tyr197. |
Product Background |
RNF43 (E3 ubiquitin-protein ligase RNF43 or RING-type E3 ubiquitin transferase RNF43) functions as a tumor suppressor, by exerting a predominant negative feedback mechanism in the Wnt/β-catenin signaling pathway. RNF43 inhibits Wnt/beta-catenin signaling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
RNF43 |
Synonyms |
E3 ubiquitin-protein ligase RNF43;RING finger protein 43;RING-type E3 ubiquitin transferase RNF43;Rnf43 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly24-Tyr197 |
Molecular Weight |
The protein has a predicted MW of 45.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Mouse R spondin 1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA. |
|
|
Mouse RNF43 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse RNF43 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
