Mouse SEMA3A Protein (SEM-MM13A)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Tyr21-Val772

Catalog: SEM-MM13A

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Product Description Recombinant Mouse SEMA3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr21-Val772.

Product Background

The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.

Product Category

Recombinant Protein

Target

SEMA3A
Synonyms Semaphorin-3A; Semaphorin III; Sema III; Semaphorin-D; Sema D; coll-1; collapsin 1; Hsema-I; Hsema-III; MGC133243; SEMA1, Sema3A; SEMAD; SEMAIII; SEMAL; semaphorin L
Accession

Species

Mouse

Amino Acid Range

Tyr21-Val772
Molecular Weight The protein has a predicted MW of 86.6 kDa same as Tris-Bis PAGE result.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl, 100mM L-arginine, 100mM glutamic acid (pH 8.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Mouse SEMA3A is greater than 95% as determined by SEC-HPLC.
Mouse SEMA3A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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