Mouse SEMA4B Protein (SEM-MM14B)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Leu31-Glu703

Catalog #SEM-MM14B

Price

$280⁵⁰

Size

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Mouse SEMA4B Protein (SEM-MM14B) Summary

Product Description

Recombinant Mouse SEMA4B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu31-Glu703.

Product Background

Semaphorin 4B (SEMA4B) inhibits the invasion of non-small cell lung cancer (NSCLC) through PI3K-dependent suppression of MMP9 activation. SEMA4B may induce FoxO1 nuclear retention through suppressing PI3K/Akt signaling pathway, which subsequently inhibited cell growth through the direct nuclear target of FoxO1, p21. A role of SEMA4B in suppressing NSCLC growth, besides its role in inhibiting cell metastasis, and highlights SEMA4B as a promising therapeutic target for NSCLC.

Mouse SEMA4B Protein (SEM-MM14B) Specifications/Details

Protein	SEMA4B
Synonyms	Semaphorin-4B; Semaphorin-C; SEMA4B; KIAA1745; SEMAC; MGC131831; SemC
Accession	Q62179
Species	Mouse
Biotinylated	no
Amino Acid Range	Leu31-Glu703
Molecular Weight	The protein has a predicted MW of 75.7 kDa. Due to glycosylation, the protein migrates to 78-83 kDa based on Bis-Tris PAGE result.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse SEMA4B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse SEMA4B is greater than 95% as determined by SEC-HPLC.