Name: Mouse SIRP alpha/CD172a Protein (SRP-MM172)
Brand: KactusBio
SKU: SRP-MM172-100UG
Price: 297.0 USD
Availability: InStock

Mouse SIRP alpha/CD172a Protein (SRP-MM172)

Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).

Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test).

Mouse SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Lys32-Asn373

Catalog #SRP-MM172

Size

100UG

500UG

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Product Description	Recombinant Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys32-Asn373.
Product Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	SIRP alpha/CD172a
Synonyms	CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
Accession	BAA13520.1
Species	Mouse
Biotinylated	no
Amino Acid Range	Lys32-Asn373
Molecular Weight	The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
	Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test).
	Mouse SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC.