Mouse SIRP alpha/CD172a Protein (SRP-MM172)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys32-Asn373
Catalog #SRP-MM172
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Product Description
 Recombinant Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys32-Asn373.
Product Background
 Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Product Category
 Recombinant Protein
Target
 SIRP alpha/CD172a
Synonyms
 CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Lys32-Asn373
Molecular Weight
 The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test).
Mouse SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC.

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