Mouse SIRP alpha/CD172a Protein (SRP-MM172)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys32-Asn373
Catalog #SRP-MM172
Regular price $297.00
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Product Description

Recombinant Mouse SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys32-Asn373.

Product Background

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

SIRP alpha/CD172a

Synonyms

CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Lys32-Asn373

Molecular Weight

The protein has a predicted MW of 39 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). The affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200). Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test). Immobilized Mouse SIRP alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD47, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA (QC Test).
Mouse SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse SIRP alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC. The purity of Mouse SIRP alpha is greater than 95% as determined by SEC-HPLC.

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