- Express System: HEK293
- Product Tag: N-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Mouse SOST, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gln24-Tyr211
Product Description |
Recombinant Mouse SOST/Sclerostin Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln24-Tyr211. |
Product Background |
SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. |
Product Category |
Recombinant Protein |
Target |
SOST/Sclerostin |
Synonyms |
sclerostin; SOST; VBCHsclerosteosis; CDD; DAND6; SOST1; VBCH |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln24-Tyr211 |
Molecular Weight |
The protein has a predicted MW of 22 kDa. Due to glycosylation,the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse SOST, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 15.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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