Mouse SPARC Protein (SPA-MM101)
Mouse SPARC Protein (SPA-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ala18-Ile302
Catalog #SPA-MM101
Regular price $300.00
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Product Description
Recombinant Mouse SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile302.
Product Background
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
Product Category
Recombinant Protein
Target
SPARC
Synonyms
SPARC; BM-40; ON
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala18-Ile302
Molecular Weight
The protein has a predicted MW of 33.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SPARC is greater than 95% as determined by SEC-HPLC.

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