Product Description |
Recombinant Mouse SPARC Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala18-Ile302. |
Product Background |
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed. |
Product Category |
Recombinant Protein |
Target |
SPARC |
Synonyms |
SPARC; BM-40; ON |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala18-Ile302 |
Molecular Weight |
The protein has a predicted MW of 33.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse SPARC on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse SPARC is greater than 95% as determined by SEC-HPLC. |
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