Mouse SPP1/OPN Protein (SPP-MM101)
Mouse SPP1/OPN Protein (SPP-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu17-Asn294
Catalog #SPP-MM101
Regular price $375.00
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Product Description

Recombinant Mouse SPP1/OPN Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu17-Asn294.

Product Background

Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.

Product Category

Recombinant Protein

Protein

SPP1/OPN

Synonyms

Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Leu17-Asn294

Molecular Weight

The protein has a predicted MW of 31.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse SPP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse SPP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse SPP1 is greater than 95% as determined by SEC-HPLC. The purity of Mouse SPP1 is greater than 95% as determined by SEC-HPLC.

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