Mouse TIGIT Protein (TIG-MM110) Summary
Product Description |
Recombinant Mouse TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly26-Thr143. |
Product Background |
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Mouse TIGIT Protein (TIG-MM110) Specifications/Details
Protein |
TIGIT |
Synonyms |
TIGIT; VSIG9; VSTM3; WUCAM |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly26-Thr143 |
Molecular Weight |
The protein has a predicted MW of 14 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 4.00 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse CD155, His Tag immobilized on CM5 Chip can bind Mouse TIGIT, His Tag with an affinity constant of 4.00 uM as determined in SPR assay (Biacore T200). |
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Mouse TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse TIGIT is greater than 95% as determined by SEC-HPLC. |
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