Mouse TIGIT-Protein (TIG-MM210)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA See testing image for detail.
  • Amino Acid Range: Gly26-Thr143

Catalog: TIG-MM210

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Product Description Recombinant Mouse TIGIT-Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly26-Thr143.

Product Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Product Category

Recombinant Protein

Target

TIGIT
Synonyms TIGIT; VSIG9; VSTM3; WUCAM
Accession

Species

Mouse

Amino Acid Range

Gly26-Thr143
Molecular Weight The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA
The purity of Mouse TIGIT-is greater than 95% as determined by SEC-HPLC.
Mouse TIGIT-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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