Mouse TIGIT-Protein (TIG-MM210)
Mouse TIGIT-Protein (TIG-MM210)
Mouse TIGIT-Protein (TIG-MM210)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA See testing image for detail.
  • Amino Acid Range: Gly26-Thr143
Catalog #TIG-MM210
Regular price $297.00
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Product Description

Recombinant Mouse TIGIT-Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly26-Thr143.

Product Background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

TIGIT

Synonyms

TIGIT; VSIG9; VSTM3; WUCAM

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Gly26-Thr143

Molecular Weight

The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA Immobilized Mouse CD155, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 56.5ng/ml determined by ELISA
Mouse TIGIT-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse TIGIT-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse TIGIT-is greater than 95% as determined by SEC-HPLC. The purity of Mouse TIGIT-is greater than 95% as determined by SEC-HPLC.

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