Mouse TIGIT-Protein (TIG-MM210)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse CD155, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly26-Thr143

Catalog: TIG-MM210

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Product Description	Recombinant Mouse TIGIT-Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly26-Thr143.
Product Background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Product Category	Recombinant Protein

Target	TIGIT
Synonyms	TIGIT; VSIG9; VSTM3; WUCAM
Gene/Protein ID	NP_001139797.1
Species	Mouse
Exact Sequence	Gly26-Thr143
Molecular Weight	The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 48-57 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse CD155, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Mouse CD155, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Mouse TIGIT, hFc Tag with the EC50 of 0.23ug/ml determined by ELISA.
	The purity of Mouse TIGIT-is greater than 95% as determined by SEC-HPLC.
	Mouse TIGIT-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.