Mouse TNFRSF11A/Rank Protein (RNK-MM211)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val31-Ser214

Catalog: RNK-MM211

$315.00
Size:

Order now get it on

Product Description Recombinant Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val31-Ser214.

Product Background

TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.

Product Category

Recombinant Protein

Target

TNFRSF11A/Rank
Accession

Species

Mouse

Amino Acid Range

Val31-Ser214
Molecular Weight The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.
The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Mouse TNFRSF11A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in