Product Description |
Recombinant Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Val31-Ser214. |
Product Background |
TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas. |
Product Category |
Recombinant Protein |
Target |
TNFRSF11A/Rank |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val31-Ser214 |
Molecular Weight |
The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Mouse RANKL, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA. |
|
|
Mouse TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC. |
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