• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
  • Amino Acid Range: Val31-Ser214
Catalog #RNK-MM211
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Mouse TNFRSF11A/Rank Protein (RNK-MM211) Summary

Product Description

 Recombinant Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val31-Ser214.

Product Background

 TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.

Mouse TNFRSF11A/Rank Protein (RNK-MM211) Specifications/Details

Protein
 TNFRSF11A/Rank
Synonyms
 ODFR; Receptor activator of NF-KB; NF-KB; NFKB; CD265; TNFRSF11A; Rank; FEO; LOH18CR1; OPTB7; OSTS; PDB2; TRANCER;
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Val31-Ser214
Molecular Weight
 The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Mouse TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC. The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...